Reaction sequence and molecular mass of a Cl(-)-translocating P-type ATPase.
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چکیده
منابع مشابه
Stoichiometry of a Cl(-)-translocating ATPase.
It has been determined that Mg2+ stimulates phosphorylation while Cl- stimulates dephosphorylation of the chloride pump during its reaction sequence. The stoichiometry of ATP hydrolyzed to Cl- transported during a single cycle of the reaction sequence was ascertained. Intracellular concentrations of ATP, ADP and inorganic phosphate were determined and, coupled with an estimate of the standard f...
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The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli. The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype c...
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چکیده ندارد.
CtpA, a copper-translocating P-type ATPase involved in the biogenesis of multiple copper-requiring enzymes.
The ctpA (ccoI) gene product, a putative inner membrane copper-translocating P1B-type ATPase present in many bacteria, has been shown to be involved only in the cbb(3) assembly in Rhodobacter capsulatus and Bradyrhizobium japonicum. ctpA was disrupted in Rubrivivax gelatinosus, and the mutants showed a drastic decrease in both cbb(3) and caa(3) oxidase activities. Inactivation of ctpA results a...
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Large single three-dimensional crystals of the dodecylmaltoside complex of the Neurospora crassa plasma membrane H(+)-ATPase (H(+) P-ATPase) can be grown in polyethylene-glycol-containing solutions optimized for moderate supersaturation of both the protein surfaces and detergent micellar region. Large two-dimensional H(+) P-ATPase crystals also grow on the surface of such mixtures and on carbon...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1993
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.90.17.7970